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| Registros recuperados : 18 | |
| 5. |  | SILVA, J. M. F; RWAHNIH, M. A.; BLAWID, R.; NAGATA, T.; FAJARDO, T. V. M. Discovery and molecular characterization of a novel enamovirus, Grapevine enamovirus-1. Virus Genes, v.53, n. 4, p. 667-671 [online], 2017.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 6. | | KAUFFMANN, C. M.; BOARI, A. de J.; SILVA, J. M. F.; BLAWID, R.; NAGATA, T. Complete genome sequence of mimosa mosaic virus, a new sobemovirus infecting Mimosa sensitiva L. Archives of Virology, v. 168, n. 1, Article number 28, Jan. 2023.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 7. | | KAUFFMANN, C. M.; BOARI, A. de J.; SILVA, J. M. F.; BLAWID, R.; NAGATA, T. Complete genome sequence of patchouli chlorosis-associated cytorhabdovirus, a new cytorhabdovirus infecting patchouli plants in Brazil. Archives of Virology, v. 167, n. 12, p. 2817-2820, 2022.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 8. | | SILVA, J. M. F.; FAJARDO, T. V. M.; AL RWAHNIH, M.; BLAWID, R.; NAGATA, T. Molecular characterization of grapevine enamo-like virus, a novel putative member of the genus enamovirus. Virus Reviews and Research, v. 20, n. 2, p. 27, 2016. (Annals of the XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology). Annals of the XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology, Pirienópolis, GO, 2016.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 9. | | SILVA, J. M. F.; FAJARDO, T. V. M.; AL RWAHNIN, M.; BLAWID, R.; NAGATA, T. Molecular characterization of Grapevine enamolike virus, a novel putative member of the genus Enamovirus. Virus Reviews and Research, n. 20, v. 2, p. 28-29, 2016. (Annals of the XXVII Brazilian Congress of Virology & X Mercosur Meeting of Virology). Annals of the XXVII Brazilian Congress of Virology & X Mercosur Meeting of Virology, Pirenópolis, GO, 2016.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 10. | | SILVA, J. M. F.; BLAWID, R.; NAGATA, T.; FAJARDO, T. V. M. In silico detection of a defective genomic RNA of Grapevine leafroll-associated virus 4 strain 5 in High-Throughput Sequencing data. In:Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), 19., 2018, Santiago, Chile. Anais... santiago, Chile: ICGV, April, 2018. p. 162-163.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 12. | | SILVA, J. M. F.; PEREIRA, B. R. de M.; AI RWAHNÍH, M.; BLAWID, R.; NAGATA, T.; FAJARDO, T. V. M. Discovery and partial characterization of a novel virus, tentatively named Grapevine virgalike virus. In:Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine (ICVG), 19., 2018, Santiago, Chile. Anais... santiago, Chile: ICGV, April, 2018. p. 146-147.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 13. | | KAUFFMANN, C. M.; BOARI, A. de J.; SILVA, J. M. F.; BLAWID, R.; KITAJIMA, E. W.; NAGATA, T. Detection, characterization, and occurrence of black pepper virus F in black pepper plants in Brazil. Tropical Plant Pathology, v. 48, n. 3, p. 347-351, 2023.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 16. | | SOUZA, E. B.; NICKEL, O.; NAGATA, T.; SILVA, J. M. F.; FAJARDO, T. V. M.; BARROS, D. R. Recombination events in full genome sequences of apple stem grooving virus. Virus Reviews and Research, v. 20, n. 2, p. 146, 2016. (Annals of the XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology). Annals of the XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology, Pirienópolis, GO, 2016.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 17. | | DUARTE, M. F.; ANDRADE, I. A. de; SILVA, J. M. F.; MELO, F. L. de; MACHADO, A. M.; INOUE-NAGATA, A. K.; NAGATA, T. Metagenomic analyses of plant virus sequences in sewage water for plant viruses monitoring. Tropical Plant Pathology, v. 48, p. 408-416, 2023.| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 18 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
05/02/2024 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 4 |
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Autoria: |
DUARTE, M. F.; ANDRADE, I. A. de; SILVA, J. M. F.; MELO, F. L. de; MACHADO, A. M.; INOUE-NAGATA, A. K.; NAGATA, T. |
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Afiliação: |
MACÁRIA FERREIRA DUARTE, UNIVERSIDADE DE BRASÍLIA; IKARO ALVES DE ANDRADE, UNIVERSIDADE DE BRASÍLIA; JOÃO MARCOS FAGUNDES SILVA, UNIVERSIDADE DE BRASÍLIA; FERNANDO LUCAS DE MELO, UNIVERSIDADE DE BRASILIA; ANA MARIA MACHADO, COMPANHIA DE SANEAMENTO AMBIENTAL DO DF; ALICE KAZUKO INOUE NAGATA, CNPH; TATSUYA NAGATA, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Metagenomic analyses of plant virus sequences in sewage water for plant viruses monitoring. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 48, p. 408-416, 2023. |
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DOI: |
10.1007/s40858-023-00575-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Frequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources. The potential use of sewage for a virus survey is discussed in this study. MenosFrequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources.... Mostrar Tudo |
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Palavras-Chave: |
High-throughput sequencing. |
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Thesagro: |
Vírus. |
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Thesaurus NAL: |
Wastewater. |
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Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 02294naa a2200241 a 4500
001 2161643
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008 2023 bl uuuu u00u1 u #d
024 7 $a10.1007/s40858-023-00575-8$2DOI
100 1 $aDUARTE, M. F.
245 $aMetagenomic analyses of plant virus sequences in sewage water for plant viruses monitoring.$h[electronic resource]
260 $c2023
520 $aFrequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources. The potential use of sewage for a virus survey is discussed in this study.
650 $aWastewater
650 $aVírus
653 $aHigh-throughput sequencing
700 1 $aANDRADE, I. A. de
700 1 $aSILVA, J. M. F.
700 1 $aMELO, F. L. de
700 1 $aMACHADO, A. M.
700 1 $aINOUE-NAGATA, A. K.
700 1 $aNAGATA, T.
773 $tTropical Plant Pathology$gv. 48, p. 408-416, 2023.
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